Journal: Frontiers in Medicine
Article Title: Gastrodia elata fermentation alleviates methamphetamine-induced neuroinflammation and anxiety- and depression-like behaviors by regulating the PI3K-AKT signaling pathway
doi: 10.3389/fmed.2026.1768944
Figure Lengend Snippet: FGE mitigates METH-induced neuronal loss in the mouse hippocampus. (A) Representative immunohistochemical images of NeuN expression in the hippocampus CA1, CA3, and DG regions across different groups. Red arrows indicate examples of pyknotic or damaged neurons. (B–D) Quantitative analysis of the number of NeuN-positive cells in the hippocampus CA1, CA3, and DG regions. * p < 0.05, ** p < 0.01, *** p < 0.001; # p < 0.05, ## p < 0.01, ### p < 0.001, & p < 0.05, && p < 0.01, &&& p < 0.001.
Article Snippet: SP Rabbit/Mouse HRP Kit (DAB) (Cat# SA00001-2/SA00001-1, CWBIO, China); DAB Substrate Kit (Cat# ZLI-9018, Zhongshan Jinqiao, Beijing, China); SPARKscript II RT Kit/SYBR Green RT-PCR Kit (Cat# AG0305-B/AH0104-B, SparkJade, China); Anti-Iba1 Rabbit Polyclonal Antibody (Cat# F155306, Abways, China); Anti-GFAP Rabbit Polyclonal Antibody (Cat# CY5424, Abcam, United Kingdom); Anti-NeuN Rabbit Polyclonal Antibody (Cat# 26975-1-AP, Proteintech, China); AKT/phosphorylated AKT (p-AKT)/phosphorylated PI3K (p-PI3K)/GAPDH (Cat# 10176-2-AP/66444-1-IG/20584-1-AP/60004-1-IG, Proteintech, China); PI3K (Cat# AF3242, Affinity, China).
Techniques: Immunohistochemical staining, Expressing